investigation of enzyme immobilization effects on its characteristics

background: enzymes are well known as sensitive catalysts in the laboratory and industrial scale. to improve their properties and for using their significant potential in various reactions as a useful catalyst the stability of enzymes can often require improvement. enzymes immobilization on solid supports such as epoxy- functionalized ferric silica nanocomposite can be effective way to improve their characteristics. methods: in this study silica coated magnetite nanoparticles were functionalized with gptsm as a linker, then immobilization reaction performed by using  various amounts of lipase b from candida antarctica (calb), for the next step immobilization effects on thermal stability and optimum ph were investigated in comparison with free calb. results: resultsillustrated enzyme was successfully immobilized on nano particles and immobilized derivative retains 100% of its activity by 55°c while free calb loss its activity at the same condition. conclusion: immobilization of calb on fe3o4@sio2 particles resulted in significant improvements in its characteristics such as thermal stability and methanol tolerance compared to the free calb. , then immobilization reaction performed by using  various amounts of lipase b from candida antarctica (calb), for the next step immobilization effects on thermal stability and optimum ph were investigated in comparison with free calb.  results:results illustrated enzyme was successfully immobilized on fe3o4@sio2 particles and immobilized derivative retains 100% of its activity by 55°c while free calb loses its activity at the same condition. also results proved  optimum ph for both form of enzyme were 7.5. conclusion: immobilization of calb on fe3o4@sio2 particles resulted in significant improvements in its characteristics such as  thermal stability and methanol tolerance  compared to the free calb.